The Mitra Project

7-Hydroxymitragynine

7-OH · CAS 174418-82-7

Indole alkaloid

Formula

C₂₃H₃₀N₂O₅

Molecular Weight

414.5 g/mol

Abundance in Leaf

≤2%

Last Reviewed

2026-03-12

What Is 7-Hydroxymitragynine?

7-Hydroxymitragynine (7-OH) is a trace alkaloid in kratom leaves, typically making up less than 2% of total alkaloid content. Despite its low concentration, it is roughly 13 times more potent than morphine at the mu-opioid receptor and is considered a major contributor to kratom's analgesic effects. It is formed partly through oxidation of mitragynine, both in the plant and during human metabolism (CYP3A4 converts mitragynine to 7-OH in the liver). Like mitragynine, 7-OH is a G-protein-biased agonist — it preferentially activates pain-relief signalling while producing less beta-arrestin-2 recruitment than classical opioids, which may translate to reduced respiratory depression and constipation. If you see "7-OH" on a Certificate of Analysis, this is the compound being measured. Regulatory attention has focused on 7-OH due to its potency, and several states have enacted concentration limits for it in consumer products.

Dose-Dependent Effects

As part of whole-leaf kratom

  • Contributes significantly to analgesia even at low leaf doses
  • At higher leaf doses, increases sedation and euphoria
  • Effects are modulated by co-occurring alkaloids (especially mitragynine)

As reported in Kruegel & Grundmann 2018; Matsumoto et al. 2004, not a recommendation.

Receptor Activity

ReceptorKi (nM)Activity TypeWhat This Means
Mu-opioid (MOR)~13Full agonist (G-protein biased)Potent activation of pain-relief receptors — roughly 13x more potent than morphine. Shows G-protein bias, meaning it preferentially activates analgesic signalling over beta-arrestin pathways linked to respiratory depression.
Kappa-opioid (KOR)Weak activityMinimal interaction with kappa receptors, which are associated with dysphoria and hallucinations.
Delta-opioid (DOR)AntagonistBlocks delta-opioid receptors, a profile shared with mitragynine and several other kratom alkaloids.

Safety & Adverse Effects

Potency warning

7-OH is significantly more potent than mitragynine at the mu-opioid receptor. Concentrated or synthetic 7-OH products carry higher risk of adverse effects including respiratory depression, especially when combined with other CNS depressants.

Source: Kruegel & Grundmann 2018; FDA advisory 2021

Dependence potential

Due to its high mu-opioid potency, 7-OH may contribute disproportionately to dependence development from regular kratom use, despite being present in low concentrations in leaf material.

Source: Matsumoto et al. 2005; Hemby et al. 2019

Regulatory status

Several U.S. states have enacted concentration limits for 7-OH in consumer kratom products under the Kratom Consumer Protection Act framework. Some jurisdictions treat concentrated 7-OH products differently from whole-leaf kratom.

Source: AKA KCPA model legislation

Drug Interactions

CYP3A4 inhibitors (ketoconazole, grapefruit juice)

major

7-OH is produced from mitragynine via CYP3A4. Inhibitors may alter the mitragynine-to-7-OH conversion ratio, potentially affecting potency and duration.

CNS depressants (benzodiazepines, alcohol, opioids)

major

Additive sedation and respiratory depression risk. The high mu-opioid potency of 7-OH makes this combination particularly dangerous.

MAOIs

major

Theoretical risk of serotonin syndrome and unpredictable potentiation of opioid effects. Avoid combination.

COA Connection

Appears on Certificates of Analysis as: 7-OH. Graded under P_acc (Potency Accuracy) (Key safety metric).

View graded products

Cited Literature

[1]

Kruegel AC, Grundmann O (2018). The medicinal chemistry and neuropharmacology of kratom: A preliminary discussion of a promising medicinal plant and analysis of its potential for abuse. ACS Chem Neurosci.

DOI: 10.1021/acschemneuro.7b00579
[2]

Váradi A, Marrone GF, Palmer TC, et al. (2016). Mitragynine/corynantheidine pseudoindoxyls as opioid analgesics with mu agonism and delta antagonism, which do not recruit β-arrestin-2. J Am Chem Soc.

DOI: 10.1021/jacs.6b00360
[3]

Matsumoto K, Horie S, Ishikawa H, et al. (2004). Antinociceptive effect of 7-hydroxymitragynine in mice: Discovery of an orally active opioid analgesic from the Thai medicinal herb Mitragyna speciosa. Life Sci.

DOI: 10.1016/j.lfs.2003.10.006
[4]

Hemby SE, McIntosh S, Leon F, et al. (2019). Abuse liability and therapeutic potential of the Mitragyna speciosa (kratom) alkaloids mitragynine and 7-hydroxymitragynine. Addict Biol.

DOI: 10.1111/adb.12639